Inquiry of Water-Soluble Polysaccharide Extraction Conditions from Grapefruit Skin

Abstract

Water-soluble polysaccharide was isolated from grapefruit with the method of water-dissolving and ethanol-precipitating. By single factor test and orthogonal experimental method, the extraction conditions of the wa-ter-soluble polysaccharide were investigated, which contained liquid-solid ratio, temperature, time and pH. The results showed that pH was the most important factor of polysaccharide extraction, followed by tem-perature. The optimized extraction technology conditions were obtained. The liquid-solid ratio was 1:40, temperature was 90˚C, time was 2.0 h, and pH was 2. The highest extraction rate of crude polysaccharide was 8.30%.