The Effect of Some Heavy Metals on the Growth of Garlic under In Vitro Conditions

Abstract

Garlic (Allium sativum L.) is an important plant species because of its nutritional and medical value. One of the important advantages of plant tissue culture is in vitro selection. The tolerance of garlic to some heavy metals under in vitro conditions was studied. In vitro experiments were carried out in Murashige and Skoog (MS) nutrient medium supplemented with different doses of cadmium (Cd), cobalt (Co), nickel (Ni), aluminum (Al), copper (Cu), chromium (Cr), and lead (Pb) at 100 mm, 200 mm, 300 mm, 400 mm, and 500 mm. During experiments, plant length, number of leaves, leaf length, root number, and root length parameters were considered. In the findings, there was a decrease in both leaf and root development as doses of heavy metals increased. In addition, it has been found that the decrease in plant development at doses of 400 mm and 500 mm was higher compared with other doses. There was no root formation at high doses of Cd, Co, Ni, and Cu (400 mm and 500 mm). As a recommendation, further research should be conducted under in vivo conditions to evaluate the level of tolerance to heavy metals in garlic.