Studies on Phosphorylation. I. Phosphorylation of Nucleosides with Organic Amine Salts of Phosphoric Acid

Abstract

Inosine 5ʹ-phosphate has been synthesized by heating isopropylidene-inosine with mono (or di)(tri-n-butylammonium)-, mono (triethylammonium)-, or monoanilinium phosphate in dimethylformamide followed by removal of the protecting group. Similarly, from isopropylidene-adenosine, -guanosine, -cytidine and -uridine, the corresponding 5ʹ-nucleotides were obtained and rom thymidine, its mono- and di-phosphate. Furthermore, inosine 5ʹ-phosphate and 5ʹ-diphosphate were prepared by heating isopropylidene inosine with tri-n-butylammonium-pyrophosphate or -polyphosphate. © 1966, The Pharmaceutical Society of Japan. All rights reserved.