Silver Nanoparticle Produced by Using Abelmoschus esculentus (L.) Moench Leaves Extract Bioreduction Processs as Blood Glucose Nanosensor

Abstract

Silver nanoparticles were produced through bioreduction using Abelmoschus esculentus leaves extract and applied as a blood glucose nano sensor. The biosynthetic reaction produced silver nanoparticles by mixing Abelmoschus esculentus leaves extract and Ag+. The formation of silver nanoparticles was characterized by a color change in the solution from yellow to brown. Silver nanoparticles were analyzed using UV-Vis, FT-IR, PSA, and TEM. Furthermore, the design and testing of blood glucose nanosensors was carried out. The UV-Vis test results showed that the best silver nanoparticles were produced during an incubation period of 6 days with a band gap energy of 2.096 eV. The FT-IR spectrum showed that there had been a bioreduction process as indicated by a decrease in the intensity of the functional groups which were bioreductors. TEM and PSA results showed that silver nanoparticles were spherical and oval in shape with a size of less than 50 nm. The design and application results for the nano sensor showed that silver nanoparticles were in the range of 0.5 mM - 8 mM with a Regression (R) of 0.9494. The detection limit of the silver nanoparticle sensor was at a concentration of 1.68 mM with a sensitivity of 3.27 A. mM-1. mm-2. The glucose level contained in blood samples with silver nanoparticle sensors was 93.05 mg / dL with a measurement difference of 1.95% when compared to Automated Analyzed Clinical Chemistry as gold standard.