Preparation and characterization of silk fibroin from four different species of Thai-local silk cocoon for Bone implanted applications

Abstract

The metal interlocking nail is normally used in diaphyseal fractures. The bio-composite interlocking nail will be fabricated in the near future by using local silk fibroin reinforce Polylactic acid filament for 3D printing. Four species of local Thai Bombyx mori silk cocoons were selected to extract silk fibroin. The silk cocoon species consist of 1) Nangnoi Srisaket-I (NN), 2) Nanglai (NL), 3) Luang Saraburi (LS), and 4) J108. After the extraction, fibroins of each silk cocoon species were characterized and compared the physical property by using Scanning Electron Microscopy (SEM), Energy Dispersive X-ray (EDS) and Fourier Transform Infrared Spectroscopy (FT-IR). Then, the biological test was performed on cell viability and cytotoxicity with human fetal osteoblast cell line. The result presents that all of local silk cocoons species presented non-cytotoxicity ability which can be used in human or animal body without endangerment.