A genetically-encoded photosensitiser demonstrates killing of bacteria by purely endogenous singlet oxygen

Abstract

TagRFP, a fluorescent protein capable of photosensitizing the production of singlet oxygen, was expressed in E. coli. Subsequent exposure to green light induced bacterial cell death in a light-dose dependent manner. It is demonstrated for the first time that intracellular singlet oxygen is sufficient to kill bacteria. © 2012 The Royal Society of Chemistry and Owner Societies.