Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells

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Abstract

Calcineurin is an evolutionarily conserved, ubiquitously expressed protein phosphatase that serves as a major effector of Ca 2+ signals, regulating diverse biological processes such as gene expression, tissue differentiation, immune responses, and neural plasticity. The following method describes how to monitor real-time calcineurin activity in cultured mammalian cells using a fluorescence resonance energy transfer (FRET)-based activity reporter. © 2014 Springer Science+Business Media, LLC.

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Mehta, S., & Zhang, J. (2014). Using a genetically encoded FRET-based reporter to visualize calcineurin phosphatase activity in living cells. Methods in Molecular Biology, 1071, 139–149. https://doi.org/10.1007/978-1-62703-622-1_11

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