Cytogenetic investigations in wheat, rye and triticale. I. evaluation of improved giemsa C-and fluorochrome banding techniques in rye chromosomes

Abstract

Giemsa’s solution and the fluorescent agents Hoechst 33258, DAPI and quinacrine were applied to chromosomes of rye in order to obtain reliable banding techniques. Methodical improvements led to distinct Giemsa C-banding patterns revealing a high number of interstitial bands which allow the discrimination even between closely related rye forms. Hoechst 33258 performed a clear fluorescent banding pattern in rye chromosomes while DAPI showed a similar but less bright fluorescence. Quinacrine proved to be an inadequate tool for rye cytogenetics, although in interphase nuclei regions of brighter fluorescence appeared. Comparing Giemsa C-and Hoechst 33258 banding, the latter could serve for a rapid identification of rye chromosomes, however, Giemsa C-banding is the method at choice for detailed analysis. © 1988, The Genetical Society of Great Britain.