Ultrabright bimetallic AuAg complex: From luminescence mechanism to biological application

Abstract

Metal clusters have attracted wide interests due to their unique electronic and optical properties, but the low luminescence quantum yield (QY) prevents them from potential biomedical applications. In this work, silver-doped Au nanoclusters (NCs) are shown to be able to improve the QY of metal clusters. We succeeded in synthesizing ultrabright glutathione (GSH) protected AuAg clusters with 10.8% QY by a one-pot route. Their florescence is about 7.5 times brighter than pure Au NCs, with super photostability and good biocompatibility in physiological environment. Based on density functional theory (DFT) calculations, we investigated the electronic structures and optical properties of the AuAg NCs. The results show that the increase of the density of states of the lowest unoccupied molecular orbital (LUMO) leads to the fluorescence enhancement. In addition, two-photon excitation fluorescence imaging has been performed to show their great potential for biomedicine.