Analysis of molecular weight albumin concentrate on various types of freshwater fish using SDS-page electrophoresis method

Abstract

This study aims to determine the distribution of molecular weight distribution in the extraction results of freshwater fish albumin that will be compared with Snakehead fish to see the similarity of distribution. The samples used were 7 types of fish obtained from the waters around Makassar. Fish albumin concentrates were analyzed for molecular weights using the SDS-PAGE electrophoresis method. Running SDS-PAGE is done at 100-120 V, for 60-90. The gel reading is done using a solution coomassie blue. The results showed that Three spot gourami fish had the most protein bands, which were 8 bands. Snakehead fish has 7 protein bands. Catfish, Tilapia, Pangas Catfish, and Common carp fish have 6 protein bands. Eels have the least protein bands, which are 4 bands. Judging from the protein composition in each type of fish, Three spot gourami fish have a complete protein and are similar to Snakehead fish.