Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis

105Citations
Citations of this article
152Readers
Mendeley users who have this article in their library.

Abstract

Recently, there has been a rapid progress in the development of techniques for isothermal amplification of nucleic acids as an alternative to polymerase chain reaction (PCR). The advantage of these methods is that the nucleic acids amplification can be carried out at constant temperature, unlike PCR, which requires cyclic temperature changes. Moreover, isothermal amplification can be conducted directly in living cells. This review describes the principles of isothermal amplification techniques and demonstrates their high efficiency in designing new highly sensitive detection methods of nucleic acids and enzymes involved in their modifications. The data on successful application of isothermal amplification methods for the analysis of cells and biomolecules with the use of DNA/RNA aptamers are presented.

Cite

CITATION STYLE

APA

Bodulev, O. L., & Sakharov, I. Y. (2020, February 1). Isothermal Nucleic Acid Amplification Techniques and Their Use in Bioanalysis. Biochemistry (Moscow). Pleiades journals. https://doi.org/10.1134/S0006297920020030

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free