Effects of Brewer Grain Meal with Enzyme Combination on Growth Performance, Nutrient Digestibility, Intestinal Morphology, Immunity, and Oxidative Status in Growing Pigs

Abstract

This study investigated the effects of supplementing feed with various levels of brewer grain meal (BGM) and enzymes (amylase, xylanase, β-glucanase, lipase, cellulase, β-mannanase, phytase, and pectinase) on growth performance, nutrient digestibility, intestinal morphology, immunity, and oxidative status in growing pigs. Eighty growing pigs were subjected to four feed treatments (five replicates per treatment), based on a corn-soybean basal diet: feeds with 0.1% enzyme combination supplementation (PC), no enzyme supplementation (NC), 20% BGM with 0.1% enzyme combination (BGM20), and 40% BGM with 0.1% enzyme combination (BGM40). Supplementing the feed with both BGM-supplemented diets significantly increased final body weight, average daily gain, the digestibility of crude protein and ash, serum concentration of total proteins, superoxide dismutase activity, villus height in the duodenum and jejunum, and duodenal villus height to crypt depth ratio; however, it did not significantly increase blood urea nitrogen, tumor necrosis factor-alpha, malondialdehyde levels, and duodenal crypt depth compared to the NC diet (p < 0.05). Furthermore, a lower hindgut pH in the middle of the colon was detected following the BGM-supplemented diet compared to PC treatment (p = 0.005). Increased levels of triglycerides and albumin were detected in BGM20-fed pigs, whereas increased levels of glucose, total antioxidant capacity, and glutathione peroxidase but decreased interleukine-6 levels were observed in the BGM40 compared with the NC group (p = 0.05). No differences were observed in the average daily feed intake and gain to feed ratio, in the serum levels of aspartate aminotransferase or immunoglobulins (p > 0.05). The addition of up to 40% BGM combined with 0.1% enzyme supplementation positively promotes the growth performance, nutrient utilization, and intestinal health of growing pigs.