Seminal analysis, cryogenic preservation, and fertility in matrinxã fish, Brycon cephalus (Günther, 1869)

Abstract

Aiming to improve fish reproduction techniques, the characterization and cryopreservation of semen of Brycon cephalus were performed. The seminal characteristics observed were: an almost transparent, milky semen with a mean volume of 4mL, and sperm concentration of 9.617±1.630 × 10 6 spermatozoa/mm3. Spermatozoa (length = 31.288 ±4.47 μm) were of the aquasperm type and displayed a small, round head (length = 1.727 ±0.18 μm; width = 1.752 ±0.17 μm) without acrosomal vesicle, nucleus with highly condensed chromatin forming coarse clots and centriolar complex located in the nuclear fossa; a midpiece (length = 2.561 ±0.44 μm), narrowed rearward, with a cytoplasmic canal; and a flagellum (length = 29.521 ±4.37 μm). Fertilization tests with thawed semen demonstrated a significant effect (α= 0.05) on the increase of thawed semen fertility with diluent type B in both 0.5mL and 4.0 mL straws. No significant effect (α= 0.05) on hatching rate was observed in both straw sizes used.