Journal Article

Quantitative determination of allantoin in biological fluids by reversed-phase high-pressure liquid chromatography

Analytical Biochemistry (1982) 123(1) 11-13
DOI: 10.1016/0003-2697(82)90616-9
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Abstract

Allantoin is quantitatively determined in biological fluids by reversed-phase high-pressure liquid chromatography. The proteins of blood plasma are precipitated by perchloric acid. Urine can be analyzed directly. The reproducibility was over 99%. The average recovery of added allantoin in blood was 96% and in urine 98%. The retention factor k′ was 0.235. © 1982.

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Tiemeyer, W., & Giesecke, D. (1982). Quantitative determination of allantoin in biological fluids by reversed-phase high-pressure liquid chromatography. Analytical Biochemistry, 123(1), 11–13. https://doi.org/10.1016/0003-2697(82)90616-9

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