A chemoenzymatically synthesized cholesterol-gpoly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer

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Abstract

Background: An amphiphilic cationic copolymer cholesterol-g-poly(amine-co-ester), namely Chol-g-PMSC-PPDL synthesized in a chemoenzymatic route has been utilized as a carrier for p53 gene delivery to check its antitumor efficacy, using human prostate cancer cell line PC-3 (p53 null) as a model. Materials and methods: The transfection efficiency was measured by quantitative PCR and Western blotting assay. The anti-proliferative effect was detected using MTT method, colony formation assay and Live/Dead staining. The anti-migration effect was evaluated through wound healing and Transwell migration assays. Results: The transfection efficiency assay indicated that the carrier-mediated p53 gene transfection could dramatically enhance the intracellular p53 expression level. Through p53 gene delivery, obvious anti-proliferative effect could be detected which was elucidated to be associated with the simultaneous activation of mitochondrial-dependent apoptosis pathway and cell cycle arrest at G1 phase. Meanwhile, the anti-migration effect could be obtained after p53 gene transfection. Conclusion: Chol-g-PMSC-PPDL-mediated p53 gene transfection could potentially be employed as a promising strategy for achieving effective anti-tumor response.

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APA

Dong, M., Chen, J., Zhang, J., Liang, X., Yang, J., Li, D., & Li, Q. (2019). A chemoenzymatically synthesized cholesterol-gpoly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer. International Journal of Nanomedicine, 14, 1149–1161. https://doi.org/10.2147/IJN.S191905

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