A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols

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Abstract

The flavin-dependent monooxygenase, HadA, catalyzes the dehalogenation and denitration of the toxicants, nitro- and halogenated phenols, to benzoquinone. The HadA reaction can be applied in one-pot reactions towards the de novo synthesis of d-luciferin by coupling with d-Cys condensation. d-luciferin, a valuable chemical widely used in biomedical applications, can be used as a substrate for the reaction of firefly luciferase to generate bioluminescence. As nitro- and halogenated phenols are key indicators of human overexposure to pesticides and pesticide contamination, the technology provides a sensitive and convenient tool for improved biomedical and environmental detection at ppb sensitivity in biological samples without the requirement for any pre-treatment. This dual-pronged method combines the advantages of waste biodetoxification to produce a valuable chemical as well as a smart detection tool for environmental and biomedical detection.

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Watthaisong, P., Pongpamorn, P., Pimviriyakul, P., Maenpuen, S., Ohmiya, Y., & Chaiyen, P. (2019). A Chemo-Enzymatic Cascade for the Smart Detection of Nitro- and Halogenated Phenols. Angewandte Chemie - International Edition, 58(38), 13254–13258. https://doi.org/10.1002/anie.201904923

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