Antimicrobial activity of Leuconostoc mesenteroides biofilm against different microorganisms

Abstract

Back ground: Leuconostoc is one of the species of lactic acid bacteria that produced biofilms.Probiotic bacteria that produced biofilm  has  been used as naturopathy  against different microbial pathogens. Objective: This study was conducted to determine the antimicrobial activity of  Leuconostoc  biofilm , against 24 isolates (4 of 6 different types) of food borne pathogens including Staphylococcus aureus , Salmonella spp, Escherichia coli , Klebsiella spp , Pseudomonas aeruginosa , Streptococcus mutans , Bacillus subtillus , Bacillus cereus, Bacillus sterothermophillus and Candida albicans. Materials and methods: using various concentration in vitro by filter paper disk diffusion method . Result: The present study showed the potent antimicrobial activity of the Leuconostoc mesenteroides biofilm against the all tested bacterial pathogens except  Bacillus species and yeast Candida albicans. Biofilm produced by Leuconostoc mesenteroides  showed highest zone of inhibition (13mm) against  Escherichia coli  and lowest zone of inhibition (7.0mm) against Streptococcus mutans . Conclusion: Consequently, Leuconostoc mesenteroides biofilm may be used as an antimicrobial agent in food products to prevent spoilage.