Enzymatic extraction of potato starch: A parametric optimization study using response surface methodology

Abstract

Optimized, effective and efficient methodology has been determined in this research work for the recovery of starch from potatoes. Potato starch extraction experimental results have been utilized for the parametric optimization study by using different statistical techniques. In this research work, starch extraction was conducted by employing cellulase enzyme. Response surface methodology (RSM) was put to use to perform statistical analysis to get optimum results. Five-level central composite design (CCD) consisting of three parameters was implemented to investigate the effect of enzyme concentration, contact time and broth dilution. Experiment results revealed that increment in enzyme concentration and contact time enhanced the starch recovery while dilution showed the inverse relation on the recovery of starch. Optimum starch recovery was achieved upto 89% when enzyme concentration (0.5 g/100 g) of potato meal was diluted with 10 mL of water and mixed for 4 h at 45°C.