Biosorption of lead and cadmium and the potential role of Shewanella oneidensis

Abstract

The bacterium Shewanella oneidensis was isolated from the soil in southern of Iraq,and identified basis on the traditional tests (morphological and biochemicaly ) and development way( high equrance gene sequence using 16S rRNA gene phylogeny analysis). The results, indicated that the isolates bacterium was Shewanella oneidensis. For emphasis the bioremediation ability of bacteria, advance techniques has been applied, included: Fourier Transform Infrared (FTIR), X-ray diffusion analysis (XRD) and broadcast electron microscope (TEM). Biosorption using different concentration from Cd+2 and Pb+2 with different times has been used to clarify the bioremediation ability. The results of TEM indicate the presence of these metals around the cells of this bacterium with the appearance of some morphological changes. The results of FTIR, and XRD showed a different shifting on the peaks of spectra when they compare with the normal one with occur different peaks for loading bacteria with studied metals, these changes can emphasize the absorption ability of these bacteria.