Nanoparticle aggregate-based fluorescence enhancement for highly sensitive and reproducible detection of DNA

Abstract

(Graph Presented) Sensitive detection of DNA at the sub picomolar range is demonstrated using a magnetic bead sandwich hybridization assay coupled with surface-enhanced fluorescence (SEF)-based amplification. Unlike enzymatic amplification, the SEF amplification step does not add any additional background to the measurement and does not cause a significant increase in the coefficient of variance (CV).