Electrochemical characterisation and application of multi microelectrode array devices to biological electrochemistry

Abstract

A new design for microelectrode array (MEA) devices fabricated by semiconductor-processing techniques is presented. The microelectrode surfaces consist of gold and are surrounded by an insulating silicon nitride layer. Each chip of these so-called Multi MEAs contains regular arrays with circular-shaped electrodes of eight different sizes: 1, 3, 5, 10, 50, 100, 500 and 1000μm. The Multi MEAs were electrochemically characterised by use of ferrocenecarboxylic acid. Well-defined cyclic voltammograms of the two small redox proteins, horse heart cytochrome c and amicyanin from Thiobacillus versutus, were obtained at variously surface-modified Multi MEAs. Furthermore, a very simple method to manufacture Multi MEAs with carbon surfaces is introduced. © 2001 by MDPI.