Chromosomal location of eight isozyme loci in rice using primary trisomics and monosomic alien addition lines

Abstract

Starch-gel electrophoresis and isoelectric focusing techniques were used to detect polymorphism and chromosomal location of isozyme loci in rice (Oryza sativa L.). Polymorphism for four new isozyme loci-Mdh-3, Gdh-2, Fk- 1, and Fk-2-was detected. Primary trisomic analysis showed that Dia-1 and Fk- 1 are located on chromosome 1, Mdh-1 on chromosome 5 and Fk-1, and Gdh-2 and Tpi-1 as a cluster of genes on chromosome 11. Monosomic alien addition line analysis revealed that Mdh-3 and Pgd-2 are located on chromosomes 5 and 6, respectively.