The antibacterial activities of bacteriocin Pediococcus acidilactici of breast milk isolate to against methicillin-resistant Staphylococcus aureus

Abstract

Resistance has occurred if an antibiotic cannot kill pathogenic bacteria therefore an antibiotic therapeutic effect cannot be achieved. Broad and free use of antibiotics effected in health problems, one of the health problems is bacteria are experienced the resistance such as Methicillin-Resistant Staphylococcus aureus (MRSA). Therefore an antibiotic alternative is needed from natural ingredients have the potential as anti-MRSA such as bacteriocin. This research was conducted to determine the antibacterial activity of bacteriocin from Pediococcus acidilactici of breast milk isolate (ASI) against MRSA. Bacteriocin isolation was carried out by growing of P.acidilactici on the media of Rogosa Sharpe (MRS) broth. Antibacterial activity test was carried out by diffusion method are to see the inhibition zone and dilution to determine the value of Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC). The test results shown that bacteriocin P.acidilactici strains A11 and C12 strains had antibacterial activity against MRSA. The A11 strain inhibition zone is 15 mm, MIC and MBC values are 25%. While the C12 strain inhibition zone produced 18 mm, the value of MIC and MBC was 12.5%.