NF-κB inhibits sodium transport via down-regulation of SGK1 in renal collecting duct principal cells

Abstract

Tubulointerstitial inflammation is a common feature of renal diseases. We have investigated the relationship between inflammation and Na+ transport in the collecting duct (CD) using the mCCDcl1 and mpkCDDcl4 principal cell models. Lipopolysaccharide (LPS) decreased basal and aldosterone-stimulated amiloride-sensitive transepithelial current in a time-dependent manner. This effect was associated with a decrease in serum and gucocorticoid-regulated kinase 1 (SGK1) mRNA and protein levels followed by a decrease in epithelial sodium channel (ENaC) α-subunit mRNA levels. The LPS-induced decrease in SGK1 expression was confirmed in isolated rat CD. This decreased expression of either SGK1 or the ENaC α-subunit was not due to enhanced degradation of mRNA. In contrast, LPS inhibited transcriptional activity of the SGK1 promoter measured by luciferase-reporter gene assay. The effect of LPS was not mediated by inhibition of mineralocorticoid or glucocorticoid receptor, because expression of both receptors was unchanged and blockade of either receptor by spironolactone or RU486, respectively, did not prevent the down-regulation of SGK1. The effect of LPS was mediated by the canonical NF-κB pathway, as overexpression of a constitutively active mutant, IKKβ (inhibitor of nuclear factor κB kinase-β) decreased SGK1 mRNA levels, and knockdown of p65 NF-κB subunit by small interfering RNA increased SGK1 mRNA levels. Chromatin immunoprecipitation showed that LPS increased p65 binding to two NF-κB sites along the SGK1 promoter. In conclusion, we show that activation of the NF-κB pathway down-regulates SGK1 expression, which might lead to decreased ENaC α-subunit expression, ultimately resulting in decreased Na+ transport. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.