Modulatory effects of N-acetyl-L-cysteine on human eosinophil apoptosis

Abstract

Eosinophils are oxidant-sensitive cells considered relevant in allergic inflammation. The present study aimed to examine the effects of the antioxidant N-acetyl-L-cysteine (NAC) on constitutive and cytokine-delayed apoptosis in human isolated eosinophils. Human eosinophils were purified from the blood of healthy donors by a magnetic separation system. Apoptosis and cellular glutathione were assessed by cytofluorometric analysis and nuclear factor (NF)-κB binding activity assessed by electrophoresis mobility shift assay. The rate of spontaneous apoptosis of human eosinophils after 24 h culture, as assessed by annexin-V-positive staining, was mean±sem 48.2±1.4%, n=5. Granulocyte-macrophage colony-stimulating factor (GM-CSF; 10 ng·mL-1) decreased apoptosis to 19.4±1.8%, n=5. NAC (5 mM) inhibited spontaneous apoptosis (33.6±2.7%, n=5) but augmented apoptosis in the presence of GM-CSF (30.9±1.5%, n=5). NAC (5 mM) also increased the rate of apoptosis in the presence of tumour necrosis factor (TNF)-α (10 ng·mL-1) and interleukin-5 (5 ng·mL-1). NAC (5 mM) increased eosinophil glutathione content. The increase in eosinophil NF-κB binding activity induced by GM-CSF and TNF-α was suppressed by NAC. In conclusion, N-acetylcysteine modulates eosinophil apoptosis by inhibiting constitutive apoptosis but reversing the survival effect produced by inflammatory cytokines in human eosinophils. Copyright©ERS Journals Ltd 2007.