Two pathways for store-mediated calcium entry differentially dependent on the actin cytoskeleton in human platelets

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Abstract

A major pathway for stimulated Ca2+ entry in non-excitable cells is activated following depletion of intra-cellular Ca2+ stores. Secretion-like coupling between elements in the plasma membrane (PM) and Ca 2+ stores has been proposed as the most likely mechanism to activate this store-mediated Ca2+ entry (SMCE) in several cell types. Here we identify two mechanisms for SMCE in human platelets activated by depletion of two independent Ca2+ pools, which are differentially modulated by the actin cytoskeleton. Ca2+ entry induced by depletion of a 2,5-di-(tert-butyl)-1,4-hydroquinone (TBHQ)-sensitive pool is increased by disassembly of the actin cytoskeleton and that induced by a TBHQ-insensitive pool is reduced. Stabilization of the actin cytoskeleton prevented Ca 2+ entry by both mechanisms. We propose that the membrane-associated actin network prevents constitutive Ca2+ entry via both pathways. Reorganization of the actin cytoskeleton permits the activation of Ca 2+ entry via both mechanisms, but only SMCE activated by the TBHQ-insensitive pool requires new actin polymerization, which may support membrane trafficking toward the PM.

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Rosado, J. A., López, J. J., Harper, A. G. S., Harper, M. T., Redondo, P. C., Pariente, J. A., … Salido, G. M. (2004). Two pathways for store-mediated calcium entry differentially dependent on the actin cytoskeleton in human platelets. Journal of Biological Chemistry, 279(28), 29231–29235. https://doi.org/10.1074/jbc.M403509200

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