Study on the influencing conditions in the electro-transformation efficiency of Bacillus licheniformis ATCC14580

Abstract

In current study, the plasmid pWB980 isolated from Bacillus licheniformis ATCC14580 was used to research the influencing conditions in electro-transformation efficiency of B. licheniformis ATCC14580 by changing the parameters of the high osmolarity electroporation method. The restriction modification system (RMS) of B. licheniformis was the main influence factor on the electro-transformation efficiency of B. licheniformis. Meanwhile, the glycine which was added in growth media resulted in a significant increase in the transformation efficiency of B. licheniformis. When the glycine concentration of the growth media was 7.5 mg/ml, the bacterial concentration OD600 1.2-1.4 and the voltage was 21 kV/cm, a maximum value of 1.3 × 104 transformats per lg pWB980 was obtained. The results indicated that glycine was useful in improving the electro-transformation efficiency of B. licheniformis. © Springer-Verlag Berlin Heidelberg 2014.