Simultaneous determination of naproxen and diflunisal using synchronous luminescence spectrometry

Abstract

Binary mixtures of naproxen and diflunisal can be resolved by using zero-crossing first derivative emission spectrofluorimetry, first derivative constant wavelength synchronous luminescence spectrometry and first derivative constant energy synchronous luminescence spectrometry. These methods do not require any previous separation steps. The lowest quantitation limits for both drugs were obtained with first derivative constant wavelength synchronous luminescence spectrometry (0.002 and 0.015 μg ml -1 for naproxen and diflunisal, respectively). The measurements were performed in 40% methanolic aqueous medium at pH 8.0 provided by adding 0.02 M phosphate buffer solution. The proposed methods were successfully applied to the simultaneous determination of naproxen and diflunisal in pharmaceuticals and human serum samples with high precision and accuracy. Linearity, accuracy, precision, limits of detection, limits of quantitation, and other aspects of analytical validation are included in the text. © 2008 Springer Science+Business Media, LLC.