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Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes.

Nucleic acids research. Supplement (2001) (2003) (3) 265-266
DOI: 10.1093/nass/3.1.265
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Abstract

Effective photo-regulation of transcription reaction by SP6 RNA polymerase (RNAP) was achieved with photo-responsive SP6 promoter tethering two azobenzenes. With one azobenzene in either TATA or RNAP binding region of the SP6 promoter, photo-regulation activity was very small. But when two azobenzenes were introduced into both regions, efficient photo-regulation of transcription was attained: transcription proceeded 3.5 fold faster under UV irradiation than under dark.

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Liu, M., Asanuma, H., & Komiyama, M. (2003). Effective photo-regulation of transcription reaction by SP6 RNA polymerase with modified DNA tethering multiple azobenzenes. Nucleic Acids Research. Supplement (2001), (3), 265–266. https://doi.org/10.1093/nass/3.1.265

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