ArcB1, a homolog of Escherichia coli ArcB, regulates dimethyl sulfoxide reduction in Shewanella oneidensis MR-1

Abstract

Shewanella oneidensis is a metal reducer that uses the cyclic AMP receptor protein, CRP, to regulate anaerobic respiration. In addition, ArcASo is required for anaerobic growth with dimethyl sulfoxide (DMSO) and plays a role in aerobic respiration. The sensor kinase that activates ArcASo in S. oneidensis is not known. ArcB1So, a homolog of the Escherichia coli sensor kinase ArcBEc, was identified and found to be required for DMSO reductase gene expression. In combination with HptA, ArcB1So complemented an E. coli arcBEc mutant. ArcASo, ArcB1 So, and HptA appear to constitute a two-component signal transduction system that regulates DMSO reduction in S. oneidensis. Copyright © 2010, American Society for Microbiology. All Rights Reserved.