Formulasi dan evaluasi SNEDDS (Self Nanoemulsifying Drug Delivery System) ekstrak daun pepaya (Carica papaya L.) sebagai analgesik

Abstract

Background: SNEDDS was chosen because of the ability to increase the absorption of drugs with low water solubility such as active substances derived from plant extraction. Objectives: This study aimed to create an innovative dosage by utilizing new drug delivery systems in the form of SNEDDS (Self Nano-emulsifying Drug Delivery System) using the active substances of papaya leaf extract (Carica Papaya L.) that is efficacious as an anti-inflammatory. Methods: SNEDDS of Papaya (Carica papaya extract L.) was prepared through the high-energy method. Dried papaya leaves were macerated using 96% ethanol, and the extract was then evaporated using a rotary evaporator to obtain viscous extract. Four formulations of SNEDDS were produced with isopropyl myristate as the oil phase, PEG 400 as the surfactant, and tween 80 as the co-surfactant. The evaluation included particle size, % transmittance, and freeze-thaw cycles. In vivo SNEDDS anti-inflammatory activity was tested in balb-c mice induced with 1% acetic acid and then compared with mefenamic acid (NSAIDs). Results: The test results showed that all of the formulations had a particle size of <200nm and >90% transmittance. The SNEDDS anti-inflammatory activity test in vivo indicated that the percent inhibition of pain in SNEDDS of papaya leaf extract reached 94.55%. Conclusion: The SNEDDS formula of papaya leaf extract marked by F4 could fulfill the criteria of good stability parameters with the smallest particle size of 77.1 nm. The percent of pain inhibition of SNEDDS papaya leaf extract in balb-c mice induced by acetic acid was 94.55%.