Standard Operating Procedures for Plasma Collection in Clinical Research

  • Identification P
  • Harel M
  • Geiger T
N/ACitations
Citations of this article
44Readers
Mendeley users who have this article in their library.

Abstract

The variables surrounding collection and processing of blood specimens affect blood chemistry and the proteome in a way that introduces systematic changes that may be mistakenly attributed to a particular physiopathological condition. A limiting factor of current clinical proteomic studies has been the lack of accepted pre-analytical and analytical guidelines. Recent worldwide efforts have been made to standardize blood collection, processing, and storage conditions for case and control samples as part of the HUPO Plasma Proteome Project initiative. Given the complexity of the blood proteome, collection and handling present a broad range of specific pre-analytical technical challenges, from venipuncture to the use of blood derivatives, protease inhibitors, and processing specifications and storage conditions. As the areas of clinical validation of different disease states from blood-derived sources (i.e., disease biomarkers) move toward validation stages, the importance of controlled and standardized protocols is imperative. The establishment of standard operating procedures (SOPs) for the collection and processing of plasma and sera allows for systematic analysis of samples without the potential of bias and variance. This protocol is the SOP for plasma/serum use in clinical proteome research, based on the Early Detection Research Network (EDRN) and Specimen Collection and Handling Committee (SCHC) of the HUPO Plasma Proteome Project [1].

Cite

CITATION STYLE

APA

Identification, P. B., Harel, M., & Geiger, T. (2017). Standard Operating Procedures for Plasma Collection in Clinical Research, 1619, 477–486. Retrieved from http://www.osha.gov/SLTC/biologicalagents/index.html

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free