Downregulation of microRNA-100 protects apoptosis and promotes neuronal growth in retinal ganglion cells

Abstract

Background: Retinal ganglion cells (RGCs) are preferentially lost in glaucoma or optic neuritis. In the present study, we investigated the protective effect of mircoRNA 100 (miR-100) against oxidative stress induced apoptosis in RGC-5 cells. Results: Rat RGC-5 cells were cultured in plates and HO was added to induce oxidative stress. TUNEL assay and qRT-PCR showed HO induced apoptosis and up-regulated miR-100 in a dose-dependent manner in RGC-5 cells. Conversely, lentiviral-mediated miR-100 down-regulation protected HO induced apoptosis, promoted neurite growth and activated AKT/ERK and TrkB pathways through phosphorylation. Luciferase assay confirmed that IGF1R was directly regulated by miR-100 in RGC-5 cells, and siRNA-mediated IGF1R knockdown activated AKT protein through phosphorylation, down-regulated miR-100, therefore exerted a protective effect on RGC-5 apoptosis. Conclusion: Down-regulating miR-100 is an effective method to protect HO induced apoptosis in RGC-5 cells, possible associated with IGF1R regulation.