Effect of Catechin on Serum Levels of Inflammatory Cytokines, Antioxidant Enzymes Activity and DNA Oxidative Damage of Ovarian Tissue in Polycystic Ovarian Syndrome Rat Model

Abstract

Introduction: Hormonal disorders associated with oxidative stress and inflammation in ovarian tissue causing anovulation in patients with polycystic ovary syndrome (PCOS). Catechin is the most important flavonoids from green (Camellia sinensis). According to the antioxidant properties of catechin, the aim of this study was to determine the effects of catechin on serum levels of inflammatory cytokines, antioxidant enzymes activity and DNA oxidative damage of ovarian tissue in polycystic ovarian syndrome rat model. Materials & methods: In this experimental study, 24 Wistar male rats were divided into 4 equal groups (n=6) of control (saline solution, 24 days, ip), non-treated PCOS (saline solution, 24 days, ip) and PCOS treated with Catechin (50 and 100 mg/kg, 24 days, ip). Polycystic ovarian syndrome was induced by single intramuscular injection of estradiol valerate (40 mg/kg) in PCOS groups. At the end of treatment period, serum level of TNF-α, IL-1β and IL-6, also level of SOD, CAT, GPX enzymes and level of MDA and HOdG-8 in ovarian tissue were measured by ELISA. Results: Compared to non-treated PCOS group, serum level of TNF-α, IL-1β and IL-6 in groups of treated with 50 and 100 mg/kg of catechin, dose-dependent significantly decreased, level of SOD, CAT, GPX enzymes in ovarian tissue significantly increased and level of MDA and HOdG-8 was significantly decreased (p<0.05). Conclusion: Catechin is decreases serum level of cytokines, increase activity of antioxidant enzymes and decreases lipid peroxidation and DNA oxidative damage of ovarian tissue in polycystic ovarian syndrome rat model.