Genomic profiling: CDNA arrays and oligoarrays

15Citations
Citations of this article
21Readers
Mendeley users who have this article in their library.
Get full text

Abstract

The introduction of microarray technology, which is a multiplexed hybridization-based process, allows simultaneous analysis of a large number of nucleic acid transcripts. This massively parallel analysis of a cellular genome will become essential for guiding disease diagnosis and molecular profiling of an individual patient's tumor. Nucleic acid based microarrays can be used for: gene expression profiling, single-nucleotide polymorphisms (SNPs) detection, array-comparative genomic hybridizations, comparisons of DNA-methylation status, and microRNA evaluation. A multitude of commercial platforms are available to construct and analyze the microarrays. Typical workflow for a microarray experiment is: preparation of cDNA or gDNA, array construction, hybridization, fluorescent detection, and analysis. Since many sources of variability can affect the outcome of one experiment and there is a multitide of microarray platforms available, microarray standards have been developed to provide industry-wide quality control and information related to each microarray. In this chapter, we review array construction, methodologies, and applications relevant to molecular profiling. © 2012 Springer Science+Business Media, LLC.

Cite

CITATION STYLE

APA

Gorreta, F., Carbone, W., & Barzaghi, D. (2012). Genomic profiling: CDNA arrays and oligoarrays. Methods in Molecular Biology, 823, 89–105. https://doi.org/10.1007/978-1-60327-216-2_7

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free