Salmonella enterica subsp. enterica serovar reading infection in dairy cattle and buffaloes suffering from Chronic Diarrhea

Abstract

The study was aimed at dairy cattle and buffaloes presented with the history of chronic diarrhea to isolate and detect Salmonella spp. Fecal samples were collected directly from the rectum of diarrheic cattle. Isolation and identification of the microorganisms was performed and confirmed on the basis of their morphology, staining, cultural, biochemical tests and sero-typing. Salmonella positive animals presented variable degree and frequency of diarrhea. Salmonellosis was confirmed in seven per cent of cattle and buffaloes suffering from chronic diarrhea. The serotype detected in the 7 Salmonella strains was Salmonella enterica subsp. enterica serovar Reading. Salmonella isolates recovered from dairy cattle had relatively variable resistance to various antimicrobial agents. The antimicrobial susceptibility pattern of the isolates showed isolates were resistant to amoxicillin, tetracycline, ampicillin, ceftriaxone. Whereas 87.5 percent, 75.0 percent and 62.5 percent susceptible to ciprofloxacin, gentamicin and co-trimoxazole respectively. This study provides updated information on the bio-incidence and susceptibility patterns of Salmonella in dairy animals suffering chronic diarrhea. Isolation and serotyping of Salmonella enterica subsp. enterica serovar Reading is new to the study and contribute to our understanding that there is shift in increases in susceptibility of dairy herds to acquire new strains.