Radioimmunoassay and other serologic tests and their application in epidemiological work

Abstract

Various types of radioimmunoassay (RIA) have been developed for demonstration and quantification of antibodies against Mycobacterium leprae. They may be classified as follows: RIA for antibodies against one cross-reacting component of M. leprae. RIA for antibodies against M. leprae specific antigenic determinants. RIA demonstrating antibodies of a particular immunoglobulin class against M. leprae. The purpose of this paper is to present our current knowledge on these various radioimmunoassays and their informative value in human leprosy and in monitoring the development of system mycobacterial infection after experimental inoculation of armadillos. Finally, the plans for further development and tests of these assays in our laboratory will be discussed.