Development of nano particle sizing system using fluorescence polarization

Abstract

In order to measure the sizes of nanoparticles with a wide size distribution in a solvent, we developed an optical microscopy system that allows for fluorescence polarization (FP) measurement and optical observation. This system allows the evaluation of nanoparticle sizes over a wide range, because the fluorescent signal intensity is independent of changes in the nanoparticle sizes. In this paper, we describe a fundamental experiment to verify the feasibility of using this system for different sizes of nanoparticles. Copyright © (2012) by the International Measurement Federation (IMEKO).