Crystallization of pertussigen from Bordetella pertussis

Abstract

A method is described for crystallizing pertussigen from Bordetella pertussis. The crystalline material induced histamine hypersensitivity in mice at a dose of 0.5 ng of protein and leukocytosis at a dose of 100 ng and was toxic at a dose of 429 μg. The histamine-sensitizing activity and the toxicity were as high as ever reported.