Isolation, Identification, Purification and in Vitro Axenic culturing of Cyanobacterial Strains from Godavari River, Nanded, Maharashtra

Abstract

Cyanobacteria (Cyanobacteria/Cyanophytes/ Cyanoprokaryotes) are a diverse group of oxygenic photosynthetic prokaryotes. Cyanobacteria are known to produce metabolites with diverse biological activities, such as antibacterial, antifungal and antiviral, herbicides, anticancer and antiplasmodial activity. The main function of cyanobacterial microalgae is to fix the atmospheric nitrogen. In present study we have isolated and identified two cyanobacterial strains from Godavari river basin, Nanded. The culturing of axenic form of cyanobacterial strains; Microcystis sp. and Oscillatoria sp. were done by using selective medium BG-11. Antifungal agents such as Streptomycin and Flucanozole were proved effective to eradicate microbial contamination at concentration of 80μg/ml. The axenic cultures were maintained for further studies.