Inhibition on HIV-1 integrase activity and nitric oxide production of compounds from Ficus glomerata

Abstract

An ethanol Ficus glomerata wood extract and its purified components were investigated for their HIV-1 integrase (IN) and nitric oxide (NO) inhibitory activities. From bioassay-guided isolation, five compounds: β-sitosterol-D- glucoside (1), aloe-emodin (2), genistein (3), 1,3,6-trihydroxy-8-methyl- anthraquinone (4) and 3-(1-C-β-D-glucopyranosyl)-2,6-dihydroxy-5- methoxybenzoic acid (5) were isolated. Among the tested samples, at concentrations of 100 μM; compound 2 showed 31.9% inhibition of HIV-1 IN, followed by 4 (19.5%), whereas other compounds were inactive. With regard to the inhibitory effect on NO production, 3 possessed the highest activity with an IC50 value of 27.5 μM, followed by 4 (IC50 = 34.7 μM) and 2 (IC50 = 41.8 μM), respectively. This is the first time that compounds 2-5 have been isolated from Ficus glomerata.