The effect of varenicline on the development and expression of nicotine-induced behavioral sensitization and cross-sensitization in rats

Abstract

The present study focused on the evaluation of behavioral sensitization and cross-sensitization induced by nicotine and varenicline in rats. Furthermore, it examined the influence of varenicline, a partial alpha4beta2 nicotinic receptor agonist, on nicotine-induced sensitization. To assess the development of behavioral sensitization, rats were chronically treated with vehicle, varenicline (0.03-3.0-mg/kg), nicotine (0.4-mg/kg) or combinations for 5-days and locomotor activity was measured. The expression of sensitization was assessed following a withdrawal period (17-26-days). The present results confirmed previous data showing the development and expression of nicotine-induced sensitization of locomotor activity in the rat. Varenicline did not induce sensitization on its own. When varenicline and nicotine were repeatedly administered sequentially, varenicline blocked the development and expression of nicotine-induced sensitization. Acute varenicline blocked the expression of nicotine-induced sensitization in a dose-dependent manner. Acute varenicline did not significantly increase locomotor activity, nor did it attenuate nicotine-induced sensitization. However, varenicline did cross-sensitize to the effects of nicotine, and vice versa. The present study showed that varenicline produced a dose-dependent bidirectional cross-sensitization with nicotine. Taken together, these findings provide pre-clinical evidence that varenicline is able to attenuate the effects of nicotine, yet simultaneously 'substitutes' for the effects of nicotine in the rat. Longitudinal studies would be needed to see if similar effects are seen in the clinical setting, and whether such effects contribute to the actions of varenicline as a smoking cessation aid. Varenicline, a partial alpha4beta2 nicotinic receptor agonist, is approved as a smoking cessation aid. This study focused on the evaluation of behavioral sensitization and cross-sensitization induced by nicotine and varenicline in rats. It was shown that varenicline produced a dose dependent bidirectional cross-sensitization with nicotine. The findings provide pre-clinical evidence that varenicline is able to attenuate the effects of nicotine, yet simultaneously "substitutes" for the effects of nicotine which raises clinical interest.