Phenotype detection of genetic enzymes b-lactamase isolation of patients with urinary tract infections bacteria Escherichia coli

Abstract

Background: the main cause of multidrug resistant bacteria is the production of β-lactamase enzymes. The objective of this study was to determine the prevalence of genotype of Escherichia coli spp. isolated from nosocomial urinary tract infections (UTI). Methods: In a cross-sectional study from May 2019 to January 2020, mid-stream urine specimens were collected from 210 patients presented with acute urinary tract infections whom attending Al-Shahid Ghazi and Al-Hariri Hospital in Medical City/ Baghdad. Samples were cultured for E. coli isolation. Antibiotic sensitivity test and the prevalence of resistance enzyme producing gen were investigated. Results: A total of 210 E. coli were isolated, multidrug resistance strain of E coli (92.85%) was reported by this study. PCR technique indicated that 88.57% of ESBL-producing isolates possessing blaTEM, 30% blaCTX-M genes and 20.48% blaSHV genes between isolates and E. coli still the less resistant to carbapenem due to low expression of KPC gen. Conclusion: ESBLs and KPC were the higher prevalent genotype of multidrug resistant E. coli in urine sample.