Role of LM23 in cell proliferation and apoptosis and its expression durin. The testis development

Abstract

LM23, a gene expressed specifically i. The testis in a stage-specific manner, has a diverse range of functions that are important in bot. The life and death of spermatogenic cells. The aim of this study was to further investigat. The expression of LM23 i. The developing rat testis an. The biological function of LM23 in proliferation and antiapoptosis in vitro. Semiquantitative reverse transcription (RT)-PCR and real-time PCR were used to examin. The expression of LM23 in testis at different developmental stages. The results suggested that LM23 mRNA levels i. The testis increased progressively after birth. The role of LM23 in proliferation was analyzed with cell counting kit-8 (CCK8), colony-forming efficiency (CFE) and flow cytometry assays. The results indicated that ectopic expression of LM23 in 293T cells significantly promoted cell proliferation by increasing cell numbers in S phase. Several methods were used, including CCK8, annexin V and propidium iodide staining and western blotting, to determin. The role of LM23 in apoptosis. The results showed that LM23 played a protective role in H 2 O 2 -induced apoptosis of 293T cells, mediated at least in part throug. The Akt/PI3K signal pathway. Taken together, these results provide new insights int. The role of LM23 i. The development o. The testes and spermatogenesis.