Instability of trinucleotide and inverted repeats is a causative factor in the development of a number of neurological diseases, hereditary syndromes, and cancer. To understand the mechanisms that lead to repeat-induced genome destabilization it is important to identify factors that affect repeat metabolism. Here we present an approach that utilizes systematic and unbiased genome-wide screen in yeast Saccharomyces cerevisiae aimed to find genes that govern GAA/TTC and inverted repeat instability. These screens allowed for the identification of more than 30 mutants with increased fragility of both repeat motifs.
CITATION STYLE
Guo, W., & Lobachev, K. S. (2020). Genetic screens to study GAA/TTC and inverted repeat instability in saccharomyces cerevisiae. In Methods in Molecular Biology (Vol. 2056, pp. 103–112). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9784-8_6
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