EFFICACY OF DIFFERENT COLLECTION TECHNIQUES ON YIELD AND QUALITY OF EGYPTIAN BUFFALO OOCYTES.

Abstract

Buffaloes ovaries obtained from slaughterhouses were used to study the influence of the oocyte collection techniques (dissection, aspiration, slicing, and aspiration plus slicing) on the availability of oocytes quantity and quality of buffalo oocytes. The oocytes were collected aseptically from the ovaries by the four methods. In all methods, oocytes were classified into 5 classes on the basis of the morphology of compact, denuded, degenerated, expanded and partial denuded oocytes. Results showed that the oocyte recovery rate from ovaries was higher (P<0.05) by aspiration plus slicing (84.67%) and slicing (83.30%) than aspiration (72.68%), while dissection technique showed the lowest (P<0.05) oocyte recovery rate (52.04%). Percentage of cumulus oocyte complexes collected by slicing (63.17%) was higher (P<0.05) than aspiration (51.34 %), aspiration plus slicing (51.24%) and dissection (42.03%). The corresponding percentages of expanded cumulus oocytes were 29.93, 30.31, 27.55 and 32.68%, respectively (P<0.05). Such results may indicate efficacy of slicing technique as a collection method on quantity and quality of buffalo oocytes.