Genetic Diversity among Pseudomonas aeruginosa Isolated from Different Sources in Al-Diwaniyah City, Iraq Using RAPD-PCR Technique

Abstract

Pseudomonas aeruginosa is an aerobic Gram-negative bacterium which has emerged as one of the most problematic nosocomial pathogens. To characterizes P. aeruginosa strains that are widespread in patients in Al-Diwaniyah city, 30 clinical and environment samples were collected from general hospitals of Al-Diwaniyah city. Methods for isolation and identifying P. aeruginosa based upon culture methods coupled with biochemical tests, A total of 11 amplified DNA fragments ranging in size from 250 to 1 Kpb were observed using two random amplified polymorphic DNA (RAPD) primers (opX-13, op Y-04) where as22 polymorphic amplified fragments were commonly detected among the 6 p. aeuroginosa isolates and each of primer give different genetic profiles. The results shows the RAPD-PCR analysis is a good epidemiological screening method to detect genetic variation among pseudomonas aeuroginosa isolates.