Serum DLK1 During Minipuberty and Pubertal Transition in Healthy Girls and in Girls With Precocious Puberty

Abstract

Context: Delta-like non-canonical notch ligand 1 (DLK1) is negatively associated with bodyweight. DLK1 pathogenic variants cause central precocious puberty (CPP) and obesity, suggesting that DLK1 links the well-established association between higher body mass index and earlier pubertal onset. However, little is known about the trajectories of circulating DKL1 in healthy girls as well as in girls with precocious puberty. Objective: To evaluate longitudinal changes in circulating DLK1 concentrations in (1) full-term, singleton healthy infant girls, (2) healthy girls during pubertal transition, and (3) girls with CPP during treatment with gonadotropin-releasing hormone agonist (GnRHa). Methods: Three longitudinal studies of (1) healthy infant girls (n = 85), (2) healthy peripubertal girls (n = 15), and (3) girls with CPP before and after GnRHa treatment (n = 15). Body fat percentage calculated using the Slaughter equation, and serum concentrations of DLK1 using enzyme-linked immunosorbent assay. Results: Serum concentration of DLK1 in healthy infant girls declined significantly through the first year of life (17.6 to 9.9?ng/mL, P =. 020). DLK1 was inversely correlated with birth weight and BF%: r = -0.220, P =. 044, and r = -0.503, P