Camel Whey Protein Attenuates Acute Heat Stress-Induced Kidney Injury in Rats by Up-Regulating CYP2J Activity and Activating PI3K/AKT/eNOS to Inhibit Oxidative Stress

Abstract

Camel whey protein is recognized for its natural antioxidant properties, attributed to its high content of antioxidant amino acids. Oxidative stress caused by heat stress poses significant harm to the body, often leading to kidney injury. This study aimed to examine the impact of camel whey protein intervention on kidney function in rats experiencing acute heat stress, as well as to explore the underlying mechanisms involved. We assessed the activity of the cytochrome P450 2J enzyme under conditions of heat stress to evaluate oxidative stress levels and kidney damage, utilizing knockout rat models. Our findings indicated that acute heat stress led to a decrease in cytochrome P450 2J expression. However, the administration of camel whey protein significantly enhanced its activity and activated associated signaling pathways. Conversely, in knockout rats, camel whey protein did not mitigate oxidative stress-related kidney damage. This research highlights the potential of camel whey protein as a protective agent against acute heat stress-induced kidney injury and the necessity of CYP2J for its protective effects.