Immunological activities of polysaccharide extracted from Elaeagnus Angustifolia l

Abstract

The study was to investigate the immunological activities of polysaccharide isolated from Elaeagnus angustifolia L. The extracted Elaeagnus angustifolia L. polysaccharide (EAP) was purified by DEAE cellulose-52 chromatography (DEAE-52) and Sephadex G-100 size-exclusion chromatography (G- 100). Then the average molecular weight (Mw) of purified polysaccharide was determined by highperformance gel permeation chromatography (HPGPC). One main fraction (EAP-1a) was obtained through the isolation and purification steps. The results showed that EAP-1a had strong immunological activities. EAP-1a could significantly increase spleen and thymus index, enhance the NK cell activity, stimulate the phagocytosis of peritoneal phagocyte and increase the level of IL-2, IFN-γ and IgG in serum. Thus, EAP-1a isolated from Elaeagnus angustifolia L. could enhance the immunological activities of immunosuppressed mice induced by cyclophosphamide (CY).